Journal
WOOD SCIENCE AND TECHNOLOGY
Volume 45, Issue 1, Pages 103-110Publisher
SPRINGER
DOI: 10.1007/s00226-010-0306-x
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Funding
- Research Council of Norway (NFR)
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The aim of this study was to use energy-dispersive X-ray spectroscopy (EDX) to localize chitosan in the cell wall of chitosan-impregnated Scots pine. It was of interest to investigate the concentration of chitosan in wood to gain further knowledge and understanding of the distribution of chitosan in the wooden matrix. After deacetylation, chitosan was re-acetylated with chloroacetic anhydride to achieve a covalent bonding of chloride to the chitosan polymer. Chloride-labelled chitosan was measured by EDX using a scanning electron microscope and described as chloride intensity. Analysis of free chloride anions was performed by dialysis and inductively coupled plasma atomic emission spectroscopy. There was a significant correlation between the molecular weight of chitosan and the intensity of covalent-bonded chloride to the chitosan polymer. High molecular weight chitosan showed a better interaction with the cell wall structure than low molecular chitosan.
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