Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for Solanum elaeagnifolium

Zhu XC, Wu HW, Raman H, Lemerle D, Stanton R & Burrows GE (2012). Evaluation of simple sequence repeat (SSR) markers from Solanum crop species for Solanum elaeagnifolium. Weed Research52, 217223. Summary Molecular markers specific for Solanum elaeagnifolium (silverleaf nightshade) are currently not available. A total of 35 simple sequence repeat (SSR) primer pairs from potato (Solanum tuberosum), tomato (S.similar to lycopersicum) and eggplant (S.similar to melongena) were tested for cross-species transferability in S.similar to elaeagnifolium. Among them, 13 primer pairs successfully produced alleles (bands). The polymorphism information content ranged from 0 to 0.84. The transferable rate of SSR from potato, tomato and eggplant to S.similar to elaeagnifolium was 20%, 40% and 46% respectively. SSR analysis revealed high level of genetic diversity among 40 individuals collected within a paddock. Highly polymorphic and transferable cross-species SSR markers would be useful for determining the extent of genetic diversity in S.similar to elaeagnifolium populations.