4.5 Article

Distribution of antibiotic resistance genes in Bosten Lake, Xinjiang, China

Journal

WATER SCIENCE AND TECHNOLOGY
Volume 70, Issue 5, Pages 925-931

Publisher

IWA PUBLISHING
DOI: 10.2166/wst.2014.321

Keywords

antibiotic-resistant bacteria; antibiotic resistance genes; quantification; sulfonamides; tetracyclines

Funding

  1. National Natural Science Foundation of China [21167014]

Ask authors/readers for more resources

The occurrence of antibiotic resistance genes (ARGs) and resistant bacteria was quantified in 17 water samples collected across Bosten Lake, Xinjiang, China. The heterotrophic plate count method was used to detect the levels of sulfonamide-and tetracycline-resistant bacteria, which have mean concentrations of 2.50 x 10(5) and 4.63 x 10(3) CFU/mL, respectively. The resistance genes of sulfonamide (sul1, sul2) and tetracycline (tetM, tetO and tetW) were detected, and results showed that all other ARGs except the tetO gene were obtained from all samples. Four of the obtained ARGs were further quantified, and results showed that the sulfonamide resistance genes were prevalent. The relative abundance was in the range of 2.81 x 10(-5) to 3.33 x 10(-3) for the sul1/16s-rRNA and 1.04 x 10(-5) to 3.80 x 10(-3) for the sul2/16s-rRNA. For the tet genes, the relative concentrations of tetM/16s-rRNA and tetW16s-rRNA ranged from 1.18 x 10(-5) to 2.46 x 10(-4) and 1.58 x 10(-6) to 4.19 x 10(-4), respectively. The concentration divergence among ARGs may be related to the different characteristics of each gene. This study validated that Bosten Lake was affected by ARGs and resistant bacteria, thus turning the lake into an important reservoir for the transfer of ARGs and resistant bacteria.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available