Journal
WATER SCIENCE AND TECHNOLOGY
Volume 69, Issue 1, Pages 120-127Publisher
IWA PUBLISHING
DOI: 10.2166/wst.2013.564
Keywords
acclimation; anaerobic granular sludge; trichloroethylene; upflow anaerobic sludge blanket reactor
Funding
- National Natural Science Foundation of China [41073071]
- programs for Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang [2012CJHB001]
- New Century Excellent Talents in University [NCET-10-0145]
- Open Project of State Key Laboratory of Urban Water Resource and Environment
- Harbin Institute of Technology [QAK201013]
- University Science and Technology Innovation Team Construction Projects of Heilongjiang Province [2013TD003]
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The granulation process was examined in an 8 L laboratory upflow anaerobic sludge blanket (UASB) reactor using synthetic wastewater contained trichloroethylene (TCE). Glucose and lactate were used as primary substrates. The anaerobic bacteria biomass were acclimated and granulated by increasing the chemical oxygen demand (COD) and TCE loadings. Anaerobic sludge was acclimated successfully in 120 days in the anaerobic sludge acclimation appliance. Since start-up, the UASB was operated as a continuous-flow reactor under the following operation conditions: temperature of (35 +/- 1) W C, pH approximate to 7.2, hydraulic retention time of 10 h, COD of 2.5 g L-1 and TCE loading rate from 50.5 to 252.3 mg center dot (Ld)(-1). The UASB reactor was started successfully. The sludge volume index was 13 mL g(-1). The maximum specific methanogenic activity was 1.42 gCOD center dot (gVSS.d)(-1). After 90 days, 85% of COD and 85% of TCE removal efficiencies were achieved. The TCE degrading granular sludge had an average diameter of 2.7mm and total suspended solid of 52 g L-1. Anaerobic sludge adsorption of TCE reached adsorption equilibrium in 0.5 h, and in 1 h reached desorption equilibrium. Furthermore, cis-dichloroethylene and vinyl chloride were detected, which showed that the removal of TCE was caused by both adsorption and biodegradation but mainly by biodegradation.
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