Journal
FRONTIERS IN PLANT SCIENCE
Volume 6, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2015.00784
Keywords
coinoculations; Bacillus simplex; genome studies; rhizosphere; legumes
Categories
Funding
- NSF [IOS 1201735]
- Shanbrom Family Foundation
- U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility [DE-AC02-05CH11231]
- Fundacion Ramon Areces (Spain)
- State Foundation for Studying Abroad of China
- NSF DUE [1022918]
- Howard Hughes Medical Institute [52006944]
- Direct For Education and Human Resources [1022918] Funding Source: National Science Foundation
- Division Of Undergraduate Education [1022918] Funding Source: National Science Foundation
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In previous work, we showed that coinoculating Rhizobium leguminosarum by. viciae 128C53 and Bacillus simplex 30N-5 onto PiSLIM sativum L. roots resulted in better nodulation and increased plant growth. We now expand this research to include another alpha-rhizobial species as well as a beta-rhizobium, Burkholderia tuberum STM678. We first determined whether the rhizobia were compatible with B. simplex 30N-5 by cross streaking experiments, and then Medicago truncatula and Melliotus alba were coinoculated with B. simplex 30N-5 and Sinorhizobium (Ensifer) meliloti to determine the effects on plant growth. Similarly, B. simplex 30N-5 and Bu. tuberum STM678 were coinoculated onto Macroptilium atropurpureum. The exact mechanisms whereby coinoculation results in increased plant growth are incompletely understood, but the synthesis of phytohormones and siderophores, the improved solubilization of inorganic nutrients, and the production of antimicrobial compounds are likely possibilities. Because B simplex 30N-5 is not widely recognized as a Plant Growth Promoting Bacterial (PGPB) species, after sequencing its genome, we searched for genes proposed to promote plant growth, and then compared these sequences with those from several well studied PGPB species. In addition to genes involved in phytohormone synthesis, we detected genes important for the production of volatiles, polyamines, and antimicrobial peptides as well as genes for such plant growth promoting traits as phosphate solubilization and siderophore production. Experimental evidence is presented to show that some of these traits, such as polyamine synthesis, are functional in B. simplex 30N-5, whereas others, e.g., auxin production, are not.
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