A sustainable, carbon neutral methane oxidation by a partnership of methane oxidizing communities and microalgae

Effluents of anaerobic wastewater treatment plants are saturated with methane, an effective greenhouse gas. We propose a novel approach to treat such effluents using a coculture of methane oxidizing communities and microalgae, further indicated as methalgae, which would allow microbial methane oxidation with minimal CO2 emissions. Coculturing a methane oxidizing community with microalgae in sequence batch reactors under continuous lightning yielded a factor of about 1.6 more biomass relative to the control without microalgae. Moreover, 55% less external oxygen supply was needed to maintain the methane oxidation, as oxygen was produced in situ by the microalgae. An overall methane oxidation rate of 171 +/- 27 mg CH4 L-1 liquid phase d(-1) was accomplished in a semi-batch setup, while the excess CO2 production was lower than 1 mg CO2 L-1 d(-1). Both nitrate and ammonium were feasible nitrogen sources for the methalgae. These results show that a coculture of microalgae and methane oxidizing communities can be used to oxidize dissolved methane under O-2-limiting conditions, which could lead to a novel treatment for dissolved methane in anaerobic effluents. (C) 2011 Elsevier Ltd. All rights reserved.