Journal
WATER RESEARCH
Volume 43, Issue 3, Pages 605-612Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2008.11.002
Keywords
Bacteriophages; Coagulation; Electrophoretic mobility; Particle size distribution; Real-time RT-PCR; Virus inactivation
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [19760368]
- Ministry of Health, Labor, and Welfare of Japan [19310801, 2007]
- Kajima Foundation's Research
- Grants-in-Aid for Scientific Research [19760368] Funding Source: KAKEN
Ask authors/readers for more resources
Differences in the behaviors of two surrogates for pathogenic waterborne viruses, F-specific RNA bacteriophages Q beta and MS2, were investigated during the coagulation process by using river water spiked with these bacteriophages. The particle size and electrophoretic mobility of Q beta and MS2 were similar, but the removal performances of infectious Q beta and MS2, as measured by a plaque forming unit (PFU) method, differed markedly during the coagulation process. The. removal ratio of the infectious Q beta concentration was approximately 2 log higher than that of the infectious MS2 concentration at all coagulant doses tested. The total Q beta and MS2 bacteriophage concentrations, which were measured by a real-time reverse transcription-polymerase chain reaction (RT-PCR) method and represented the total number of bacteriophages regardless of their infectivity, were similar after the coagulation process, suggesting that the behaviors of Q beta and MS2 as particles were similar during the coagulation process. The difference between total concentration and infectious concentration indicated that some of the bacteriophages were probably inactivated during the coagulation process. This differ Price was larger for Q beta than MS2, meaning that Q beta was more sensitive to the virucidal activity of the aluminum coagulant. Analysis of the PFU and real-time RT-PCR findings together suggested that the difference in removal performances of Q beta and MS2 during the coagulation process was probably caused by differences not in the extent of bacteriophage entrapment in the aluminum floc particles but in the sensitivity to virucidal activity of the aluminum coagulant. (C) 2008 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available