Journal
WATER AIR AND SOIL POLLUTION
Volume 225, Issue 1, Pages -Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s11270-013-1787-7
Keywords
Bacillus megaterium; CO2 biomineralization; Solubility trapping; Carbonate precipitation
Funding
- NRF
- INHA University Research Grant
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Implication of ureolytic biocalcification process as an efficient carbon dioxide sequestration technology was evaluated with Bacillus megaterium culture growing in a closed environment system. In three experimental sets containing either 35 % v/v CO2 in headspace (set 1) or 50 mM urea in solution (set 3) or both (set 2), change in headspace CO2, soluble calcium, and pH were monitored during 8 days of incubation. In absence of urea, headspace CO2 content continued to increase in set 1 (up to 48 % v/v) without any carbonate precipitation. However, carbonate precipitation were obvious in urea containing sets (sets 2 and 3) utilizing all the added calcium (25 mM) in just 2 and 6 days, respectively. No headspace CO2 could be detected in set 2 at the end of experimental period, and analysis suggested that this was majorly (>53 %) attributed to the solubility trapping phenomenon. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis revealed that the precipitates were made up of calcite and major fraction of vaterite polymorph of CaCO3.
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