Journal
VIRUS RESEARCH
Volume 256, Issue -, Pages 90-95Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2018.08.002
Keywords
Herpes simplex virus 1; miRNAs; Latency; Human trigeminal ganglia; Sequence variation
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Funding
- Croatian Science Foundation [8790]
- European Commission Marie Sklodowska-Curie Actions [618587]
- European Social Found [HR3.301-033]
- University of Rijeka
- Spanish Government [AGL2017-88702-C2-2-R]
- Strengthening the capacity of CerVirVac for research in virus immunology and vaccinology [KK.01.1.1.01.0006]
- European Regional Development Fund (ERDF)
- ERDF
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Human herpes simplex virus 1 (HSV-1) expresses numerous miRNAs, the function of which is not well understood. Several qualitative and quantitative analyses of HSV-1 miRNAs have been performed on infected cells in culture and animal models, however, there is very limited knowledge of their expression in human samples. We sequenced small-RNA libraries of RNA derived from human trigeminal ganglia latently infected with HSV-1 and Varicella zoster virus (VZV) and detected only a small subset of HSV-1 miRNA. The most abundantly expressed miRNAs are miR-H2, miRNA that regulates the expression of immediate early gene ICP0, and miR-H3 and -H4, both miRNAs expressed antisense to the transcript encoding the major neurovirulence factor ICP34.5. The sequence of many HSV-1 miRNAs detected in human samples was different from the sequences deposited in miRBase, which might significantly affect targeted functional analyses.
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