Journal
VIRUS RESEARCH
Volume 183, Issue -, Pages 1-5Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.01.012
Keywords
Mink enteritis virus; Infectious clone; Genetic marker; Rescue virus; Biological properties; Reverse genetics
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Funding
- 863 project [2011AA10A213]
- National Key Scientific Foundation [2009ZX08006-0108]
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Construction and characterization of a full-length infectious clone (pMEV) of mink enteritis virus are described. Feline kidney cells (F81) were transfected with pMEV containing an engineered BamHI site that served as a genetic marker. The rescued virus was indistinguishable from its parental virus. The availability of a MEV infectious clone will facilitate studies of viral replication and pathogenicity and will permit the elucidation of determinants of the host range of the parvovirus. (c) 2014 Elsevier B.V. All rights reserved.
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