Journal
VIRUS RESEARCH
Volume 191, Issue -, Pages 92-100Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2014.07.025
Keywords
Porcine reproductive and respiratory syndrome virus (PRRSV); Nonstructural protein 3 (Nsp3); Envelope protein (E); IFITM1; Tetherin; Interaction
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Funding
- National Key Basic Research Plan Grant from the Chinese Ministry of Science and Technology [2014CB542700]
- Key project of National Natural Science Funds from National Natural Science Foundation of China [31330077]
- earmarked fund for Modern Agro-industry Technology Research System of China from the Chinese Ministry of Agriculture [CARS-36]
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Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized to modulate the innate immune response of host. However, little is known about the effects of PRRSV infection on porcine intrinsic virus-restriction factors. This study presents the first demonstration that the nonstructural protein 3 (Nsp3) or envelope (E) protein of PRRSV interacted with porcine intrinsic virus-restriction factor IFITM1 or Tetherin. Next, in PRRSV-infected MARC-145 cells and the transfected cells with the IFITM1- or Tetherin-expressing plasmid, IFITM1 was shown to be mainly distributed perinuclear, and Tetherin was proposed to be partially removed away from cell surface. Moreover, the overexpression of IFITM1 and Tetherin were shown to have no obvious effects on the replication of PRRSV in MARC-145 cells. The Nsp3 of PRRSV was demonstrated to induce the proteasome-dependent degradation of IFITM1 upon PRRSV infection. These findings suggest that PRRSV might counteract the antiviral functions of IFITM1 and Tetherin by the interaction of the Nsp3 with IFITM1 and the E protein with Tetherin, providing a novel clue for exploring possible mechanisms associated with the evasion of PRRSV from immune recognition of host. (C) 2014 Elsevier B.V. All rights reserved.
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