Journal
VIRUS RESEARCH
Volume 169, Issue 1, Pages 13-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2012.06.024
Keywords
Porcine reproductive and respiratory; syndrome virus; Nonstructural proteins; PRRSV vaccines; T-cell epitopes
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Funding
- National Pork Board [NPB 10-115]
- PRRS CAP, USDA NIFA [2008-55620-19132]
- Konkuk University
- Nebraska Tobacco Settlement Biomedical Research Enhancement Funds
- [NIH-K08AI076609]
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Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins (NSPs) 9 and 10, both of which are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of four peptides, two from each NSP9 and NSP10 that appear to contain T-cell epitopes. Comparison of the amino acid sequence of these four peptide sequences to the analogous sequences from a diverse sample of typeII PRRSV strains indicated that these sequences are highly conserved and thus contain highly conserved T-cell epitopes. The identified epitopes may be important in the formulation of immunogens to provide broad cross-protection against diverse PRRSV strains. (C) 2012 Elsevier B.V. All rights reserved.
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