Journal
VIRUS RESEARCH
Volume 169, Issue 1, Pages 276-281Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2012.07.010
Keywords
TVBMV; Plant virus; Potyvirus; Infectious clone; Gene vector; Tobacco
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Funding
- Special Research Funds for Doctoral Program (SRFDP) from Ministry of Education, China [20080434006, 20123702110013]
- National Natural Science Foundation of China (NSFC) [30971895, 31011130031]
- Academy of Finland [1134759, 1253126]
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Tobacco vein banding mosaic virus (TVBMV, genus Potyvirus) mainly infects solanaceous plants and is of increasing economic importance in China. Here, we report sequence determination of the full-length 5'-untranslated region of TVBMV isolate HN39 and construction of an infectious clone. The resultant clone, pTVBMV, which was stabilized by introducing three introns in the P3 and Cl-encoding regions, induced similar disease symptoms and accumulated similar titers of virus in plants of Nicotiana benthamiana, Nicotiana tabacum and N. rustica as the wild type HN39 isolate. Mutation of arginine to isoleucine (R1821) or aspartic acid to lysine (D198K) in HC-Pro alleviated the symptoms of pTVBMV significantly, indicating a role of the two amino acids in regulating virulence of TVBMV. The Aequoria victoriae gene for green fluorescent protein was inserted between the NIb and CP encoding regions of pTVBMV and expressed stably in the systemically infected N. benthamiana leaves, indicating suitability of pTVBMV for expression of foreign proteins in plants. (C) 2012 Elsevier B.V. All rights reserved.
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