Journal
VIRUS RESEARCH
Volume 159, Issue 1, Pages 62-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2011.04.023
Keywords
Rice stripe virus; NSvc4; Plasmodesmatal localization; Early secretory pathway; Actomyosin motility system
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Funding
- programs for the New Century of Excellent Talents at Universities [NCET-09-0011]
- National Natural Science Foundation of China [30770090]
- Bio-oriented Technology Research Advancement Institution (BRAIN) of Japan
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Plant viruses utilize movement proteins to gain access to plasmodesmata (PD) for cell-to-cell propagation. While the NSvc4 protein of Rice stripe virus (RSV) is implicated in the passage of viruses from cell to cell, its role remains to be elucidated. We examined the mechanisms by which RSV NSvc4 is targeted to PD in cell walls. NSvc4 accumulated at PD when expressed as a fusion with yellow fluorescent protein in leaf cells of Nicotiana benthamiana. NSvc4 was targeted to PD via the endoplasmic reticulum-to-Golgi secretory pathway, and the actomyosin motility system was required for the delivery of NSvc4 to PD. Moreover, it appeared that NSvc4 utilized myosin VIII-1 rather than myosin XI for trafficking to PD. Taken together, our data reveal that the targeting of NSvc4 to PD exploits the early secretory pathway and the actin-myosin VIII motility system in the leaves of a non-host plant, N. benthamiana. (C) 2011 Elsevier B.V. All rights reserved.
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