Open reading frame 60 of the Bombyx mori nucleopolyhedrovirus plays a role in budded virus production

Open reading frame 60 (bm60) of the Bombyx mod nucleopolyhedrovirus (BmNPV) is a conserved gene among group I and some group II NPVs. bm60 encodes a late expressed protein that localizes to both the cytoplasm and nucleus of infected cells. This paper describes the characterization of a BmNPV mutant (vbm60-Null) lacking functional bm60. It was observed that the production of budded virus (BV) was reduced by nearly an order of magnitude relative to wt virus in vbm60-Null-infected BmN cells and B. mori larvae. Quantitative real-time PCR assay showed that the viral DNA replication was affected in infected cells due to disruption of bm60. Larval bioassays showed that the speed of kill of vbm60-Null virus was greatly reduced, as it took approximately 28-36h longer to kill the fifth instar B. mod larvae. These results suggest that BmNPV bm60 is not essential for viral replication, but required for efficient By production. (C) 2010 Elsevier B.V. All rights reserved.