Journal
VIRUS RESEARCH
Volume 145, Issue 1, Pages 106-111Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2009.06.015
Keywords
Baculovirus; DNA replication; Okazaki fragments; Mung bean nuclease
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Funding
- Russian Foundation for Basic Research [09-04-00423]
- NIH [GM9982536]
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In this report the short-lived DNA replication intermediates produced in both uninfected and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) infected Spodoptera frugiperda cells were characterized. The methods used included pulse-labeling of DNA in permiabilized cells, treatment of nascent DNA with Mung bean nuclease, and electrophoresis in neutral and alkaline agarose gels. in contrast to uninfected cells that produced a population of small DNA fragments of about 200 bp, a population of heterogeneous fragments of up to 5 kb with an average size of 1-2 kb derived randomly from the virus genome was identified as the short-lived intermediates produced during AcMNPV replication. The intermediates likely include Okazaki fragments derived from the tagging strands in viral replication forks as well as fragments produced during the recombination-dependent replication. (C) 2009 Elsevier B.V. All rights reserved.
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