Journal
VIRUS RESEARCH
Volume 137, Issue 1, Pages 147-149Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.virusres.2008.05.014
Keywords
Autographa californica multiple; nucleopolyhedrovirus; transcription initiation complex; serine/threonine kinase; very late gene expression factor; DNA affinity purification
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Funding
- Council of Scientific and Industrial Research (CSIR), India
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The baculovirus gene, protein kinase-I (pk-1) encodes a serine/threonine kinase that is essential for very late gene expression. Late and very late genes of the baculoviruses are transcribed by an alpha-amanitin resistant RNA polymerase. The very late gene promoter transcription initiation complex was isolated from nuclei of Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-infected Sf9 cells by DNA affinity purification and found to contain 4 major polypeptides of sizes similar to 102, 38, 32, and 18 kDa. The 32 kDa polypeptide was immunoreactive to AcMNPV anti-pk-1 antibody and phosphorylated the 102 kDa polypeptide, earlier reported as late gene expression factor LEF-8. Electrophoretic mobility shift assays with anti-pk-1 antibody indicated the binding of promoter DNA with recombinant AcMNPV-pk-1 and transcription initiation complex proteins. All these results suggested AcMNPV-pk-1 to be a component of the viral very late gene transcription initiation complex. (C) 2008 Elsevier B.V, All rights reserved.
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