Journal
VIRUS RESEARCH
Volume 138, Issue 1-2, Pages 7-16Publisher
ELSEVIER
DOI: 10.1016/j.virusres.2008.07.026
Keywords
Autographa californica multiple; nucleopolyhedrovirus; Baculovirus; CMV minimal promoter; Enhancer-like element; Polyhedrin upstream sequence
Categories
Funding
- Academia Sinica [94S-1303]
- National Science Council [NSC95-2313-B-001-031-MY3, NSC95-2313-B-001-032-MY3]
Ask authors/readers for more resources
Previously, we identified a novel enhancer-like element, the polyhedrin upstream (pu) sequence, in the genome of the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV), which activates several early promoters. The activation requires co-infection of AcMNPV, suggesting that viral gene products are needed for pu-mediated promoter activation. DNA replication assay showed that the pu sequence did not assist in DNA replication and suggested its involvement in activated transcription from target promoters. In order to identify the viral genes responsible for pu-dependent activation of early promoters, a set of overlapping cosmid clones covering the entire 134-kb AcMNPV genome were constructed and screened. Our results identified three viral genes ie1, ie2, and pe38, which function in concert with pu to activate target promoters. In addition, these three viral factors can substitute for the entire virus for the synergistic promoter activation mediated by pu and the known baculovirus enhancer, the homologous region. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available