Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 5, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2015.00025
Keywords
Veillonella; caries; stress proteins; pH regulation; RNA-sequencing
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Funding
- Dental Institute, King's College London
- Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre
- St Thomas' NHS Foundation Trust
- King's College London and King's College Hospital NHS Foundation Trust
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Veil/one/la spp. are predominant bacteria found in all oral biofilms. In this study, a metatranscriptomic approach was used to investigate the gene expression levels of three oral Veil/one/la spp. (V parvula, V dispar and V atypica) in whole stimulated saliva from caries-free volunteers and in carious lesions (n = 11 for each group). In the lesions the greatest proportion of reads were assigned to V parvula and genes with the highest level of expression in carious samples were those coding for membrane transport systems. All three Veillonella spp. increased expression of genes involved in the catabolism of lactate and succinate, notably the alpha- and beta-subunits of L(+)-tartrate dehydratase (EC 4.2.1.32). There was also significantly increased expression of histidine biosynthesis pathway in V parvula, suggesting higher intra-cellular levels of histidine that could provide intra-cellular buffering capacity and, therefore, assist survival in the acidic environment. Various other systems such as potassium uptake systems were also up regulated that may aid in the survival and proliferation of V parvula in carious lesions.
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