Journal
VIRUS GENES
Volume 38, Issue 3, Pages 392-397Publisher
SPRINGER
DOI: 10.1007/s11262-009-0333-6
Keywords
Multiplex PCR (mPCR); Porcine circovirus type2 (PCV2); Porcine parvovirus (PPV); Porcine pseudorabies virus (PRV); Porcine reproductive and respiratory syndrome virus (PRRSV)
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Funding
- Chinese National 10th Five-Year Science and Technology Plan [2004 BA519A01]
- National High-tech R D Program [2007AA100606]
- National Key Laboratory of Veterinary Biotechnology Fund [NKLVBP200807]
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A multiplex PCR (mPCR) assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine. Specific primers were designed for each of the following four DNA or RNA viruses: porcine circovirus type 2 (PCV2), porcine parvovirus (PPV), pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRSV). Each target produced a specific amplicon with a size of 353 bp (PCV2), 271 bp (PPV), 194 bp (PRV), or 434 bp (PRRSV). The assay was sensitive and specific in detecting each target agent in composite cell cultures and clinical specimens. Results from mPCR were confirmed by PCR for individual viruses and by virus isolation. In conclusion, the mPCR has the potential to be useful for routine molecular diagnosis and epidemiology.
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