Journal
VIROLOGY JOURNAL
Volume 8, Issue -, Pages -Publisher
BMC
DOI: 10.1186/1743-422X-8-110
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-
Categories
Funding
- Research on Emerging and Re-emerging Infectious Diseases
- Ministry of Health, Labour and Welfare, Japan
- Health and Labour Sciences Research
- Ministry of Health, Labour and Welfare of Japan
- Ministry of Education, Culture, Sports, Science and Technology, Japan [22590421]
- Kanazawa Medical University [K2010-16]
- Grants-in-Aid for Scientific Research [22590421, 22790439] Funding Source: KAKEN
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The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.
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