Journal
VIROLOGY
Volume 456, Issue -, Pages 20-27Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.01.010
Keywords
Peptides; Coronavirus; Virus attachment; PEDV
Categories
Funding
- National Natural Science Foundation of China [31340003, 31372438]
- Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang [2013CJHB002]
- Program for New Century Excellent Talents in University of Ministry of Education of P.R. China [NCET-10-0144]
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Three phage-displayed peptides designated H, S and F that recognize porcine aminopeptidase N (pAPN), the cellular receptor of porcine transmissible gastroenteritis virus (TGEV) were able to inhibit cell infection by TGEV. These same peptides had no inhibitory effects on infection of Vero cells by porcine epidemic diarrhea virus (PEDV). However, when PEDV, TGEV and porcine pseudorabies virus were incubated with peptide H (HVTTTFAPPPPR), only infection of Vero cells by PEDV was inhibited. Immunofluoresence assays indicated that inhibition of PEDV infection by peptide H was independent of pAPN. Western blots demonstrated that peptide H interacted with PEDV spike protein and that pretreatment of PEDV with peptide H led to a higher inhibition than synchronous incubation with cells. These results indicate direct interaction with the virus is necessary to inhibit infectivity. Temperature shift assays demonstrated that peptide H inhibited pre-attachment of the virus to the cells. (C) 2014 Published by Elsevier Inc.
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