Journal
VIROLOGY
Volume 442, Issue 1, Pages 3-11Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2013.03.030
Keywords
HHV-6; Human herpesvirus 6; CIHHV-6: chromosomally integrated human herpesvirus 6; PBMC; Peripheral blood mononuclear cells; Integration; Telomere; Telomere integration; single telomere length analysis; STELA
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Funding
- HHV-6 Foundation
- National Institute of Health [5R01CA111196]
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Human herpesvirus 6B (HHV-6B) is the causative agent of roseola infantum. HHV-6A and 6B can reactivate in immunosuppressed individuals and are linked with severe inflammatory response, organ rejection and central nervous system diseases. About 0.85% of the US and UK population carries an integrated HHV-6 genome in all nucleated cells through germline transmission. We have previously reported that the HHV-6A genome integrated in telomeres of patients suffering from neurological dysfunction and also in telomeres of tissue culture cells. We now report that HHV-6B also integrates in telomeres during latency. Detailed mapping of the integrated viral genomes demonstrates that a single HHV-6 genome integrates and telomere repeats join the left end of the integrated viral genome. When HEK-293 cells carrying integrated HHV-6A were exposed to the histone deacetylase inhibitor Trichostatin A, circularization and/or formation of concatamers were detected and this assay could be used to distinguish between lytic replication and latency. (C) 2013 Elsevier Inc. All rights reserved.
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