Journal
VIROLOGY
Volume 437, Issue 2, Pages 89-99Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.12.017
Keywords
Iridovirus; Megalocytivirus; Proteomics; Virus induced stress protein (VISP); Envelope protein
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Funding
- China Postdoctoral Science Foundation [201003385]
- National Basic Research Program of China [2012CB114406]
- Technology Planning Project of Guangdong Province [2011A020102002]
- Planning subject of 'the Twelfth Five-Year-Plan' in National Science and Technology for the Rural Development in China [2011BAD13B00]
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Megalocytiviruses have three main genotypes, which are represented by ISKNV, RSIV, and TRBIV. To date, the virion-associated proteins of RSIV and TRBIV are still unknown. The spotted knifejaw iridovirus (SKIV) is a newly characterized RSIV-type megalocytivirus. In this study, the virion-associated proteins of SKIV were identified by systemic one-dimensional gel electrophoresis-based proteomic approaches. A total of 49 viral proteins and 33 cellular proteins were associated with the SKIV virions by LC MS/MS, including 18 highly abundant structural proteins that were detected by MALDI TOF/TOF-MS. One highly abundant structural protein of interest was identified as the virus-inducible stress protein (VISP) and further characterized as an envelope protein. However, knockdown of mVISP by siRNA method showed no effect in virion production. The current study is the first to present detailed information on the virion-associated proteins of an RSIV-type megalocytivirus and to identify a novel cellular envelope protein of this virus. (C) 2013 Elsevier Inc. All rights reserved.
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