Journal
VIROLOGY
Volume 434, Issue 2, Pages 181-186Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.09.036
Keywords
Ocean viruses; Metagenomics; viral concentration; viral purification; Next-generation sequencing; Environmental virology; Viral ecology
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Funding
- Gordon and Betty Moore Foundation
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As new environments are studied, viruses consistently emerge as important and prominent players in natural and man-made ecosystems. However, much of what we know is built both upon the foundation of the culturable minority and using methods that are often insufficiently ground-truthed. Here, we review the modern culture-independent viral metagenomic sample-to-sequence pipeline and how next-generation sequencing techniques are drastically altering our ability to systematically and rigorously evaluate them. Together, a series of studies quantitatively evaluate existing and new methods that allow-even for ultra-low DNA samples-the generation of replicable, near-quantitative datasets that maximize inter-comparability and biological inference. (C) 2012 Elsevier Inc. All rights reserved.
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