Journal
VIROLOGY
Volume 426, Issue 2, Pages 134-142Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.01.018
Keywords
Merkel cell polyomavirus; Viral integration; Oncogenic viruses; Genetic variability; Merkel cell carcinoma
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Funding
- Institut Pasteur, the Association pour la Recherche sur le Cancer [A09/1/5041]
- La Ligue Nationale Contre le Cancer [RS10/75-1, RS11/75-63]
- Ministere de l'Enseignement Superieur et de la Recherche (MESR) of France
- Ile-de-France
- European Program EMPERIE
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Merkel cell polyomavirus (MCPyV) is associated to Merkel cell carcinoma (MCC). We studied 113 MCC tumoral skin lesions originating from 97 patients. MCPyV detection was higher in fresh-frozen (FF) biopsies (94%) than in formalin-fixed paraffin-embedded biopsies (39-47%). Mean viral load in FF tumor was of 7.5 copies per cell with a very wide range (0.01-95.4). Nineteen complete sequences of LTAg were obtained, mainly from FF biopsies when the viral load was high. Seventeen showed stop codons, all localized downstream of the pRb protein binding domain. Sequence comparison and phylogenetic analysis showed that all sequences clustered in the large C clade of MCPyV strains. MCPyV integration was demonstrated in 19 out of 27 FF MCC DNA biopsies without evidence of specific host cellular genome integration site. In 13/19 cases, the viral junction was located within the second exon of the LTAg, after the pRB binding domain. (C) 2012 Elsevier Inc. All rights reserved.
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