Journal
VIROLOGY
Volume 434, Issue 2, Pages 251-256Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.09.030
Keywords
Bacteriophage; P. putida; Exopolysaccharide-degrading enzyme; Biofilm degradation; Horizontal gene transfer
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Funding
- 'Instituut voor de aanmoediging van Innovatie door Wetenschap en Technologie in Vlaanderen' (I.W.T., Belgium)
- PhageBiotics research community (FWO Vlaanderen Grant) [WO.022.09]
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We report the study of phage AF, the first member of the canonical lambdoid phage group infecting Pseudomonas putida. Its 42.6 kb genome is related to the epsilon 15-like viruses and the BPP-1-like viruses, a clade of bacteriophages shaped by extensive horizontal gene transfer. The AF virions display exopolysaccharide (EPS)-degrading activity, which originates from the action of the C-terminal domain of the tail spike (Gp19). This protein shows high similarity to the tail spike of the T7-like P. putida-infecting phage phi 15. These unrelated phages have an identical host spectrum and EPS degradation characteristics, designating the C-terminal part of Gp19 as sole determinant for these functions. While intact AF particles have biofilm-degrading properties, Gp19 and non-infectious AF particles do not, emphasizing the role of phage amplification in biofilm degradation. (C) 2012 Elsevier Inc. All rights reserved.
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