Journal
VIROLOGY
Volume 419, Issue 1, Pages 1-9Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.07.018
Keywords
Filoviral GP; Pseudovirions; Entry; Clathrin-mediated endocytosis; Eps15; AP-2; DAB2; ARRB1; AP-1
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Funding
- National Institutes of Health (NIH) [AI052051]
- Nomis Foundation
- James B. Pendleton Charitable Trust
- Rodney Dean and Cornelia Hayes Mackey Endowment Fund
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Clathrin-mediated endocytosis was previously implicated as one of the cellular pathways involved in filoviral glycoprotein mediated viral entry into target cells. Here we have further dissected the requirements for different components of this pathway in Ebola versus Marburg virus glycoprotein (GP) mediated viral infection. Although a number of these components were involved in both cases; Ebola GP-dependent viral entry specifically required the cargo recognition proteins Eps15 and DAB2 as well as the clathrin adaptor protein AP-2. In contrast, Marburg GP-mediated infection was independent of these three proteins and instead required beta-arrestin 1 (ARRB1). These findings have revealed an unexpected difference between the clathrin pathway requirements for Ebola GP versus Marburg GP pseudovirion infection. Anthrax toxin also uses a clathrin-, and ARRB1-dependent pathway for cellular entry, indicating that the mechanism used by Marburg GP pseudovirions may be more generally important for pathogen entry. (C) 2011 Elsevier Inc. All rights reserved.
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