Journal
VIROLOGY
Volume 397, Issue 2, Pages 322-330Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.11.025
Keywords
Influenza virus; Neutralizing antibody; Phage-display Ab library; Apheresis; Hemagglutinin; Epidemics
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology
- Ministry of Health, Labor, and Welfare of Japan
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We tried to reveal the strain specificity of neutralizing mAbs against H3N2 influenza viruses in individuals. A large number of B lymphocytes of a pediatrician were collected by apheresis and two Ab libraries were constructed at 2004 and 2007 by using the phage-display technology. The libraries were screened against 12 different H3 strains Of flu isolated between 1968 and 2004. Large numbers of clones that bound to the Ags were isolated and mAbs that specifically bound to H3 strain viruses were selected. Their binding activity to the 12 strains and neutralizing activity were studied by ELISA and focus reduction test, respectively. Furthermore, the binding activity to hemagglutinin (HA) was examined by Western blot. The majority of clones showing the neutralizing activity turned out to be anti-HA mAbs and could be divided into three major groups showing distinct strain specificity: 1968-1973, 1977-1993 and 1997-2003. (C) 2009 Elsevier Inc. All rights reserved.
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