Journal
VIROLOGY
Volume 408, Issue 1, Pages 80-88Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.09.004
Keywords
Human immunodeficiency virus-1; Multivalent carbohydrates; 3 '-sialyllactose; Globotriose; Peripheral blood mononuclear cells; T cell lines
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Funding
- National Institute of Allergy and Infectious Diseases [RO1 NS40231]
- NIH, National Cancer Institute, Center for Cancer Research
- U.S. Army Medical Research and Material Command [DAMD W81XWH-04-2-0005]
- Henry M. Jackson Foundation for the Advancement of Military Medicine [DAMD W81XWH-04-2-0005]
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Specific glycosphingolipids (GSL), found on the surface of target immune cells, are recognized as alternate cell surface receptors by the human immunodeficiency virus type 1 (HIV-1) external envelope glycoprotein. In this study, the globotriose and 3'-sialyllactose carbohydrate head groups found on two GSL were covalently attached to a dendrimer core to produce two types of unique multivalent carbohydrates (MVC). These MVC inhibited HIV-1 infection of T cell lines and primary peripheral blood mononuclear cells (PBMC) by T cell line-adapted viruses or primary isolates, with IC(50)s ranging from 0.1 to 7.4 mu g/ml. Inhibition of Env-mediated membrane fusion by MVC was also observed using a dye-transfer assay. These carbohydrate compounds warrant further investigation as a potential new class of HIV-1 entry inhibitors. The data presented also shed light on the role of carbohydrate moieties in HIV-1 virus-host cell interactions. (C) 2010 Elsevier Inc. All rights reserved.
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