Journal
VIROLOGY
Volume 397, Issue 1, Pages 145-154Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.10.042
Keywords
Tetraviridae; Birnaviridae; Insect virus; Genome sequence; Permuted RNA-dependent RNA polymerase; VPg; Jelly-roll-based capsid; Phylogeny; Recombination; Taxonomy
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Funding
- New Zealand Marsden Fund [U00212]
- Netherlands Bioinformatics Center BioRange [SP 3.2.2]
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The Tetraviridae is a family of non-enveloped positive-stranded RNA insect viruses that is defined by the T=4 symmetry of virions. We report the complete Euprostema elaeasa virus (EeV) genome sequence of 5698 nt with no poly(A) tail and two overlapping open reading frames, encoding the replicase and capsid precursor, with similar to 67% amino acid identity to Thosea asigna virus (TaV). The N-terminally positioned 17 kDa protein is released from the capsid precursor by a NPGP motif EeV has 40 nm non-enveloped isometric particles composed of 58 and 7 kDa proteins. The 3'-end of TaV/EeV is predicted to form a conserved pseudoknot. Replicases of TaV and EeV include a newly delineated VPg signal mediating the protein priming of RNA synthesis in dsRNA Birnaviridae. Results of rooted phylogenetic analysis of replicase and capsid proteins are presented to implicate recombination between monopartite tetraviruses, involving autonomization of a sgRNA, in the emergence of bipartite tetraviruses. They are also used to revise the Tetraviridae taxonomy. (C) 2009 Elsevier Inc. All rights reserved.
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