Non-replicating adenovirus vectors expressing avian influenza virus hemagglutinin and nucleocapsid proteins induce chicken specific effector, memory and effector memory CD8+ T lymphocytes

Avian influenza virus (AIV) specific CD8(+) T lymphocyte responses stimulated by Intramuscular administration of an adenovirus (Ad) vector expressing either HA or NP were evaluated in chickens following ex vivo stimulation by non-professional antigen presenting cells The CD8(+) T lymphocyte responses were AIV specific, MHC-I restricted, and cross-reacted with heterologous H7N2 AIV strain. Specific effector responses, at 10 days post-inoculation (p i), were undetectable at 2 weeks p.i and memory responses were detected from 3 to 8 weeks pi Effector memory responses, detected 1 week following a booster inoculation, were significantly greater than the primary responses and. within 7 days, declined to undetectable levels Inoculation of an Ad-vector expressing human NP resulted in significantly greater MHC restricted, activation of CD8(+) T cell responses specific for AIV Decreases in all responses with time were most dramatic with maximum activation of T cells as observed following effector and effector memory responses (C) 2010 Elsevier Inc All rights reserved