Journal
VIROLOGY
Volume 387, Issue 1, Pages 235-243Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.02.025
Keywords
Prion; PMCA; Amplification; CWD; Transmissible; Spongiform; Encephalopathy; 170; Sequence; Loop; Chronic wasting disease
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Funding
- NIAID NIH HHS [N01-AI-25491] Funding Source: Medline
- NINDS NIH HHS [2R01 NS040334-04] Funding Source: Medline
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Chronic wasting disease (CWD) is an efficiently transmitted spongiform encephalopathy of cervids. Whether CWD could represent a threat to non-cervid species remains speculative. Here we show that brain homogenates from several CWD-susceptible non-cervid species, such as ferrets and hamsters, support amplification of Prp(CWD) by sPMCA, whereas brain homogenates from CWD-resistant species, such as laboratory mice and transgenic mice expressing human PrPC [Tg(HuPrP) mice], do not. We also investigated whether several North American species that share the environment with cervids Would support amplification of PrPCWD by sPMCA. Three native rodent species, including votes and field mice, supported PrPCWD amplification, whereas other species (e.g. prairie dog, coyote) did not. Analysis of PrP sequences suggests that an ability to support amplification of PrPCWD in trans-species sPMCA is correlated with the presence of asparagine at position 170 of the substrate species PrP. Serial PMCA may offer insights into species barriers to transmission of CWD. (C) 2009 Elsevier Inc. All rights reserved.
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