Journal
VIROLOGY
Volume 394, Issue 1, Pages 109-118Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.08.027
Keywords
Chimpanzee; CD4+lymphocytes; Activation; HIV-1; CCR5
Categories
Funding
- National Institutes of Health [R21 AI080364, R01 AI50529, R01 AI 58715]
- UAB Center for AIDS Research [P30 AI 27767]
- Yerkes National Primate Research Center [RR-000165]
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Human immunodeficiency virus type I (HIV-1) originated in chimpanzees: yet, several previous studies have shown that primary HIV-1 isolates replicate poorly in chimpanzee CD4+ T lymphocytes in vitro and in vivo. The reasons for this apparent restriction are not understood. Here, we describe a new activation protocol that led to a reproducible expansion and activation of chimpanzee CD4+ T lymphocytes in vitro. Using this protocol, we uncovered species-specific differences in the activation profiles of human and chimpanzee CD4+ T-cells, including HLA-DR and CD62L. Moreover, we found that improved activation facilitated the replication of both CXCR4 and CCR5-tropic HIV-1 in CD4+ T-cell Cultures from over 30 different chimpanzees. Thus, the previously reported replication block of CCR5-tropic HIV-1 in chimpanzee lymphocytes appears to be due, at least in large part, to suboptimal T-cell activation. (C) 2009 Elsevier Inc. All rights reserved.
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