Journal
VIROLOGY
Volume 388, Issue 1, Pages 1-7Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.03.024
Keywords
siRNA; Deep sequencing; Viral diagnostics; Plant viruses; Virus discovery; Sweetpotato; Badnavirus; Mastrevirus; Potyvirus; Crinivirus; Short sequence assembly
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Funding
- Howard Buffet Foundation
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We report the first identification of novel viruses, and sequence of an entire vital genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known vital pathogens, Occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge. (C) 2009 Elsevier Inc. All rights reserved.
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