Journal
VIROLOGY
Volume 387, Issue 1, Pages 5-10Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.02.039
Keywords
Hepatitis C virus; siRNA screen; Host-pathogen interaction; Lipid kinase; PI4KA
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A functional screen of an adenovirus-delivered shRNA library that targets similar to 4500 host genes was performed to identify cellular factors that regulate hepatitis C Virus (HCV) sub-genomic RNA replication. Seventy-three hits were further examined by siRNA oligonucleotide-directed knockdown, and silencing of the PI4KA gene was demonstrated to have a significant effect on the replication of a Hcv genotype 1b replicon. Using transient siRNA oligonucleotide transfections and stable shRNA knockdown clones in HuH-7 cells, the PI4KA gene was shown to be essential for the replication of all HCV genotypes tested (1a, 1b and 2a) but not required for bovine viral diarrhea virus (BVDV) RNA replication. (C) 2009 Elsevier Inc. All rights reserved.
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