Journal
VIROLOGY
Volume 378, Issue 1, Pages 118-122Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.05.019
Keywords
coxsackievirus; eIF5B; poliovirus; protease; rhinovirus; translation shutoff
Categories
Funding
- NIAID NIH HHS [R56 AI051340, R56 AI050237, R56 AI051340-06, R01 AI051340, R56 AI050237-06, AI-50237, AI-51340, R01 AI050237] Funding Source: Medline
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The enteroviruses poliovirus (PV), Coxsackie B virus (CVB) and rhinovirus (HRV) are members of Picorna-viridae that inhibit host cell translation early in infection. Enterovirus translation soon predominates in infected cells, but eventually also shuts off. This complex pattern of modulation of translation suggests regulation by a multifactorial mechanism. We report here that eIF5B is proteolytically cleaved during PV and CVB infection of cultured cells, beginning at 3 hours post-infection and increasing thereafter. Recombinant PV, CVB and HRV 3C(pro) cleaved purified native rabbit eukaryotic initiation factor (eIF) 5B in vitro at a single site (VVEQ down arrow G, equivalent to VMEQ down arrow G(479) in human eIF5B) that is consistent with the cleavage specificity of enterovirus 3C proteases. Cleavage separates the N-terminal domain of eIF5B from its essential conserved central GTPase and C-terminal domains. 3C(pro)-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells. (c) 2008 Elsevier Inc. All rights reserved.
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