Journal
VIROLOGY
Volume 372, Issue 2, Pages 340-348Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2007.10.024
Keywords
Chlorella viruses; viral K+ channel Kcv; K+ efflux; Phycodnaviridae; PBCV-1
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Funding
- NCRR NIH HHS [P20 RR15635] Funding Source: Medline
- NIGMS NIH HHS [GM32441] Funding Source: Medline
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Infection of Chlorella NC64A cells by PBCV-1 produces a rapid depolarization of the host probably by incorporation of a viral-encoded K+ channel (Kcv) into the host membrane. To examine the effect of an elevated conductance, we monitored the virus-stimulated efflux of K+ from the chlorella cells. The results indicate that all 8 chlorella viruses tested evoked a host specific K+ efflux with a concomitant decrease in the intracellular K+. This K+ efflux is partially reduced by blockers of the Kcv channel. Qualitatively these results support the hypothesis that depolarization and K+ efflux are at least partially mediated by Kcv. The virus-triggered K+ efflux occurs in the same time frame as host cell wall degradation and ejection of viral DNA. Therefore, it is reasonable to postulate that loss of K+ and associated water fluxes from the host lower the pressure barrier to aid ejection of DNA from the virus particles into the host. (c) 2007 Elsevier Inc. All rights reserved.
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