Journal
VIROLOGY
Volume 379, Issue 1, Pages 168-174Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.06.020
Keywords
Closteroviridae; crinivirus; Tomato chlorosis virus; RNA silencing suppressor
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Funding
- Plan Nacional de I+D+I,Ministerio Educacion y Ciencia, Spain [AGL2004-06959-C04-01/AGR, AGL2007-66062-C02-01/AGR]
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Viruses express proteins with silencing suppression activity to counteract the RNA silencing-mediated defense response of the host. In the family Closteroviridae, examples of multiple-component RNA silencing suppression systems have been reported. To ascertain if this is a general Strategy in this group of viruses, we have explored the bipartite genome of Tomato chlorosis virus (ToCV, genus Crinivirus). We have identified the RNA1-encoded p22 protein as an effective silencing Suppressor by using a Agrobacterium co-infiltration assay. p22 Suppressed local RNA silencing induced either by sense RNA or c1sRNA very efficiently, but did not interfere with short or long-distance systemic spread of silencing. We have also demonstrated by using the heterologous vector PVX the silencing Suppression activity of the RNA-2 encoded Coat protein (CP) and minor coat protein (Cpm). In this study, we demonstrate an even greater complexity of silencing suppressor activity for a plant Virus, and for the first time we show the presence of RNA silencing Suppressor genes encoded by both genomic RNA molecules of a bipartite genome in the complex family Closteroviridae. (c) 2008 Elsevier Inc. All rights reserved.
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