Journal
VIROLOGY
Volume 381, Issue 1, Pages 143-154Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.08.028
Keywords
HIV-1; Dendritic cells; Transmission; Cytoskeleton; Macropinocytosis; CD4+T cells
Categories
Funding
- NIH [A1068493, 1S10RR022412-01]
- Campbell Foundation
- MCW
Ask authors/readers for more resources
Dendritic cells (DCs) are among the first Immune cells to encounter HIV-1 at the initial infection. DCs efficiently transfer HIV-1 to CD4(+) T cells via infectious or virological synapses formed between DCs and T cells. Retroviruses exploit the cytoskeletal network to facilitate viral infection and dissemination however, the role of the cytoskeleton in DC-mediated HIV-1 transmission is unknown. Here, we report that intact cytoskeleton is essential for DC-mediated HIV-1 transmission to CD4(+) T cells. We found that macropinocytosis of HIV-1 contributes to DC-mediated HIV-1 endocytosis and transmission. Blocking HIV-1 macropinocytosis and disrupting actin or microtubules in DCs with specific inhibitors significantly prevented DC-mediated HIV-1 trans-infection of CD4(+) T cells. Altered HIV-1 trafficking and impaired formation of virological synapses primarily accounted for the inhibition of viral transmission by cytoskeletal inhibitors. Our results provide new insights into the mechanisms underlying DC-mediated HIV-1 transmission to CD4(+) T cells via the cytoskeletal network. published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available