Journal
VIROLOGY
Volume 378, Issue 1, Pages 34-47Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.04.037
Keywords
nuclear export; nuclear import; PRRSV; nucleocapsid protein; nucleolus; nucleus; cytoplasm; confocal; FLIP; FRAP; arterivirus; virus; nucleolar
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Funding
- Biotechnology and Biological Sciences Research Council [BB/D524875/1] Funding Source: researchfish
- BBSRC [BB/D524875/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D524875/1] Funding Source: Medline
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Porcine reproductive and respiratory syndrome Virus (PRRSV), an arterivirus, in common with many other positive strand RNA viruses, encodes a nucleocapsid (N) protein which can localise not only to the cytoplasm but also to the nucleolus in virus-infected cells and cells over-expressing N protein. The dynamic trafficking of positive strand RNA virus nucleocapsid proteins and PRRSV N protein in particular between the cytoplasm and nucleolus is unknown. In this study live imaging of permissive and non-permissive cell lines, in conjunction with photo-bleaching (FRAP and FLIP), was used to investigate the trafficking of fluorescent labeled (EGFP) PRRSV-N protein. The data indicated that EGFP-PRRSV-N protein was not permanently sequestered to the nucleolus and had equivalent mobility to cellular nucleolar proteins. Further the nuclear import of N protein appeared to occur faster than nuclear export, which may account for the observed relative distribution of N protein between the cytoplasm and the nucleolus. (c) 2008 Elsevier Inc. All rights reserved.
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