Journal
VIRAL IMMUNOLOGY
Volume 27, Issue 2, Pages 48-59Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/vim.2013.0090
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Funding
- CSIR
- Department of Biotechnology (DBT), Government of India
- DBT
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An immune role of neural stem/progenitor cells (NSPCs) has been proposed in many recent studies; however much still remains to be elucidated. In the current investigation, we report that NSPCs possess the ability to convert encephalitogenic T cells into CD4(-)(+)CD25(+)-FOXP3(+) regulatory T cells (T-reg). Encephalitogenic and nonencephalitogenic T cells isolated from sham and Japanese encephalitis virus (JEV) infected animals were co-cultured with mouse NSPCs. Post co-culture, significant increase in the number of T-regs was observed from encephalitogenic T cells co-cultured with NSPCs. This increased conversion was found to be dependent on direct contact between T cells and NSPCs. Neutralization of TGF- and IFN- in NSPC cultures abrogated this increased conversion of encephalitogenic T cells into T-regs. Flow cytometric, quantitative RT-PCR, and immunoblot analysis of both T cells and NSPCs revealed surface and intracellular changes post co-culture. Co-stimulatory molecules (B7) and ICAM-1 were increased on NSPCs post co-culture; levels of TGF, IFN, and TGFR1 were also increased in NSPCs. This study provides a basic insight into the interaction between CNS-infiltrating encephalitogenic T cells and NSPCs during viral encephalitis. Conversion of encephalitogenic T cells into CD4(-)(+)CD25(+)-FOXP3(+) T-regs through interaction with NSPCs indicates an attempt in regulation of excessive inflammation in the CNS.
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