Bioremediation of the oil spill polluted marine intertidal zone and its toxicity effect on microalgae

Custom-designed devices with 0.6 m (L) x 0.3 m (W) x 0.4 m (H) and a microbial consortium were applied to simulate bioremediation on the oil spill polluted marine intertidal zone. After the bioremediation, the removal efficiency of n-alkanes and polycyclic aromatic hydrocarbon homologues in crude oil evaluated by GC-MS were higher than 58% and 41% respectively. Besides, the acute toxicity effects of crude oil on three microalgae, i.e. Dicrateria sp., Skeletonema costatum and Phaeodactylum tricornutum, varied with concentration. The effects of microbe and surfactant treated water on the three microalgae followed a decreasing order: the microbial consortium plus Tween-80 > the microbial consortium > Tween-80. During 96 h, the cell densities of the three microalgae in treated seawater increased from 4.0 x 10(5), 1.0 x 10(5) and 2.5 x 10(5) cells per mL to 1.7 x 10(6), 8.5 x 10(5) and 2.5 x 10(6) cells per mL, respectively, which illustrated that the quality of seawater contaminated by crude oil was significantly improved by the bioremediation.