Journal
ELIFE
Volume 4, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.08649
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Funding
- National Institutes of Health [GM074207]
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Non-centrosomal microtubule arrays assemble in differentiated tissues to perform mechanical and transport-based functions. In this study, we identify Caenorhabditis elegans NOCA-1 as a protein with homology to vertebrate ninein. NOCA-1 contributes to the assembly of non-centrosomal microtubule arrays in multiple tissues. In the larval epidermis, NOCA-1 functions redundantly with the minus end protection factor Patronin/PTRN-1 to assemble a circumferential microtubule array essential for worm growth and morphogenesis. Controlled degradation of a gamma-tubulin complex subunit in this tissue revealed that.-tubulin acts with NOCA-1 in parallel to Patronin/PTRN-1. In the germline, NOCA-1 and gamma-tubulin co-localize at the cell surface, and inhibiting either leads to a microtubule assembly defect. gamma-tubulin targets independently of NOCA-1, but NOCA-1 targeting requires gamma-tubulin when a non-essential putatively palmitoylated cysteine is mutated. These results show that NOCA-1 acts with gamma-tubulin to assemble non-centrosomal arrays in multiple tissues and highlight functional overlap between the ninein and Patronin protein families.
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