Journal
ELIFE
Volume 4, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.10230
Keywords
-
Categories
Funding
- Deutsche Forschungsgemeinschaft [DFG-274/2-3/SFB655]
- Human Frontier Science Program [RGP0016/2010]
- Center for Regenerative Therapies Dresden
- DIGS -BB Program
- Agencia Nacional de Promotion Cientifica y Tecnologica [PICT-2014-3469]
- Bundesministerium fur Bildung und Forschung [0316169A]
Ask authors/readers for more resources
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available