Journal
VETERINARY PARASITOLOGY
Volume 205, Issue 1-2, Pages 113-118Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2014.06.017
Keywords
Echinococcus multilocularis; Beaver; Em2-ELISA; Em18-ELISA; Immunoblotting; EmVF-antigen
Categories
Funding
- Swiss National Science Foundation [31003A_141039/1]
- European Commission
- Swiss National Science Foundation (SNF) [31003A_141039] Funding Source: Swiss National Science Foundation (SNF)
Ask authors/readers for more resources
A novel species-specific anti-beaver-IgG-alkaline-phosphatase conjugate was synthesized for the development of a new serological test for echinococcosis in beavers. Two different ELISAs conventionally used for human Echinococcus multilocularis serology (Em18-ELISA and Em2-ELISA) yielded diagnostic sensitivities of 0% and 46%, respectively. In contrast, the subsequently developed immunoblotting assay gave an 85% diagnostic sensitivity (11 out of 13 beavers with alveolar echinococcosis were immunoblotting-positive, i.e. showed reactivity with a specific 21 Mr band), and maximal specificity. In conclusion, this immunoblotting assay should be the method of choice for use in serological studies on E. multilocularis in Eurasian beavers, and the test proved suitable to investigate both animals alive and post-mortem. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available