Journal
VETERINARY PARASITOLOGY
Volume 196, Issue 1-2, Pages 235-240Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2013.01.028
Keywords
Dirofilaria immitis; Surface associated antigens; Fibrinolysis; Plasminogen binding
Categories
Funding
- Agencia de Desarrollo Economico de Castilla y Leon, Junta de Castilla y Leon, Spain [SA090/A09]
- FEDER funds
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Cardiopulmonary dirofilariosis (Dirofilaria immitis) is characterized by apparent contradictory events, like the long-term survival of adult worms in the circulatory system of the infected hosts and the development of life-threatening events like thromboembolisms and others. Thus parasite mechanisms, like the activation of fibrinolytic system, are key to the survival of both the worms and the host. The aim of this study was to investigate the interaction between D. immitis adult worms surface-associated antigens (DiSAA) and the fibrinolytic system of the host. We demonstrate that DiSAA extract is able to bind plasminogen and generate plasmin, with the latter occurring in a tissue plasminogen activator (t-PA) dependent manner. Additionally, 11 plasminogen-binding proteins from DiSAA extract were identified by proteomics and mass spectrometry (MS) (actin-5C, actin-1, enolase, fructose-bisphosphate aldolase, GAPDH, MSP domain protein, MSP 2, beta-galactosidase-binding-lectin, galectin, immunoglobulin I-set domain-containing protein and cyclophilin Ovcyp-2). Because in a previous work we have shown the positive interaction between the excretory/secretory antigens of D. immitis (DiES) and the host fibrinolytic system and many of the molecules identified here are shared by both antigens, we hypothesize that DiSAA cooperate in host fibrinolytic system activation promoting the fibrin clot lysis. (c) 2013 Elsevier B.V. All rights reserved.
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