Journal
VETERINARY PARASITOLOGY
Volume 191, Issue 3-4, Pages 379-385Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2012.08.029
Keywords
Anti-inflammatory; Heat shock protein; Proteomics
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Funding
- BBSRC UK [BBH0092561]
- Wellcome Trust [WT094907MA]
- BBSRC [BB/K009583/1, BB/H009477/1, BB/H009256/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K009583/1, BB/H009256/1, BB/H009477/1] Funding Source: researchfish
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Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 20 electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study. (C) 2012 Elsevier B.V. All rights reserved.
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