Journal
VETERINARY PARASITOLOGY
Volume 178, Issue 1-2, Pages 64-69Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2010.12.016
Keywords
Toxoplasma gondii; Neospora caninum; Red fox; Deer; PCR; Antibodies
Categories
Funding
- Belgian Federal Public Service for Health, Food Chain Safety and Environment
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Sera from Cervidae were tested for the presence of antibodies against Neospora caninum using ELISA; and against Toxoplasma gondii using SAG1-ELISA and a commercially available agglutination test. The T. gondii seroprevalence was 52% (38/73) in roe deer (Capreolus capreolus), 0% in bred fallow deer (0/4) (Dama dama) and red deer (0/7) (Cervus elaphus). We found 2.7% of the roe deer samples and none of the bred deer samples positive for N. caninum. Brain samples from wild roe deer, red deer and red foxes (Vulpes vulpes) were tested for the presence of T. gondii and N. caninum DNA using multiplex real-time PCR. We detected T. gondii in 18.8% (57/304) of the red foxes and in 1 of the 33 deer samples. N. caninum was found in 6.6% of the red foxes and in 2 roe deer samples. Twenty-six of the T. gondii positive DNA extracts from the red fox samples were genotyped. Twenty-five were type II and only one was found to be type III. (c) 2010 Elsevier B.V. All rights reserved.
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