Journal
VETERINARY PARASITOLOGY
Volume 176, Issue 1, Pages 74-78Publisher
ELSEVIER
DOI: 10.1016/j.vetpar.2010.10.048
Keywords
Intestinal parasite; Puppy; Pet shop; Giardia; Cystoisospora; Genotype
Categories
Funding
- Veterinary Medicine of Kitasato University [S1001]
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The current study examined the prevalence of intestinal parasites and genotypes of Giardia intestinalis in puppies from nine pet shops in east Japan. Fresh fecal samples from 1794 puppies (<= 3 months old) were collected on one occasion. Giardia spp. was examined for specific coproantigen using ELISA kit (SNAP (R) Giardia, IDEXX Laboratories, Inc., USA). Other intestinal parasites were detected microscopically using the formalin-ethyl acetate sedimentation technique. Genotyping was determined for the random 29 stool samples identified as Giardia spp. positive using PCR and direct sequencing of the glutamate dehydrogenase (gdh) gene. Overall prevalence of protozoan Giardia spp. and Cystoisospora spp. revealed 23.4% and 11.3%, respectively. Prevalence of ascarids, Strongyloides spp. and hookworms were recorded 1.8%, 1.1% and 0.1%, respectively. Protozoan Giardia spp. and Cystoisospora spp., thus, represent important pathogens among pet shop puppies. All genotyped G. intestinalis isolates were belonged to assemblage C or D, identified as dog-specific genotypes. Zoonotic assemblage A and B were not demonstrated. The result suggests that the risk of zoonotic transmission of G. intestinalis from pet shops puppies to humans may be quite low in Japan. (C) 2010 Elsevier B.V. All rights reserved.
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